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The effect of temperature on the permeation of vitamin C across membranes of DPPC vesicles labelled with the spin label 1,14 has been investigated. It could be shown that at temperatures above 40 °C the permeation rate is diminished considerably despite the fact that, at these temperatures, the spin label can rotate freely (above phase transition temperature) indicating that the membrane is more fluid. The results obtained agree well with previous findings (W. Lohmann and D. Holz. Biophys. Struct. Mech. 10, 197 (1984)) according to which the furanoid ring formed by the side chain of this vitamin opens at these temperatures. The existence of a bicyclic side-chain structure seems to be a prerequisite for the permeation of vitamin C.

Notizen: Influence of Temperature on the Transport of Ascorbate across Artificial Membranes as Studied by the Spin Label Technique