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Two-dimensional gel electrophoresis according to O’Farrell [6] was used to study synthesis of membrane bound proteins during outgrowth of Bacillus subtilis spores. Three major classes of membrane bound proteins were distinguished, depending on the time of onset and duration of their synthesis: Ia - a great amount of proteins typical of vegetative cells made throughout all stages of outgrowth and vegetative growth; Ib - vegetative proteins synthesized during middle and later outgrowth stages as well as during vegetative growth (sequential activation); II - outgrowth-specific proteins synthesized during early outgrowth but not in vegetative cells. A similar classification was made for total proteins [9].

zeitschrift für naturforschung c

Untersuchungen über die Regulation der Proteinsynthese auswachsender Sporen von Bacillus subtilis / Studies on Protein Synthesis of Outgrowing Spores of Bacillus subtilis