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The effects of osmolarity, calcium concentration and cell-size liposomes in the subphase on the surface tension of phospholipid monolayers were investigated. The monolayers were spread from chloroform solutions of phosphatidic acid at air/water solution interface. The liposomes (of average diameter 3 μm) were formed from phosphatidic acid/egg lecithin (1:2) mixtures in water or 0.1 ᴍ water solutions of sucrose.

Kinetics of Calcium-Induced Fusion of Cell-Size Liposomes with Monolayers in Solutions of Different Osmolarity