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A new procedure for the isolation of highly purified acylamino acid amidohydrolase from hog kidney is d e­ scribed which allows the preparation of the enzyme with a recovery of about 45%, a 200 fold purification and a spec.activity of 350-500 U. The essential Zn 2+  of the enzyme was exchanged for Co 2+ , Ni 2+ , Mn 2+  and Cd 2+ , and the kinetic parameters K M , k cat  and k cat / K M  of the different enzyme species for a series of acetyl-L-amino acids were determined.

zeitschrift für naturforschung c

Ein neues Isolierungsverfahren für Schweinenierenacylase. Kinetik des Co 2+ , Mn 2+ , Ni 2+  und Cd 2+ -enzyms / A New Isolation Procedure for Acylamino Acid Amidohydrolase. Kinetics of the Co 2+ , Mn 2+ , Ni 2+  and Cd 2+  Enzyme