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A cell-free system from developing seeds of red radish (Raphanus sativus L. var. sativus) catalyzed the transfer of the sinapoyl moiety of 1-O-sinapoyl-β-ᴅ-glucose to choline to form O-sinapoylcholine (sinapine). A wide range of proportionality with respect to incubation time and protein concentration was found in the determination of this enzymatic activity using a high performance liquid chromatographic (HPLC) method. The transacylase activity showed an absolute acceptor specificity towards choline and a high hydroxycinnamoyl donor specificity with 1-sinapoylglucose. Maxim|al rate of sinapine formation was found to be around pH 7.0 in 0.08 M potassium phosphate buffer. The apparent K m  values were 0.30 mм for 1-sinapoylglucose and 7.64 mм for choline.

zeitschrift für naturforschung c

Enzymatic Synthesis of Sinapine from 1-O-Sinapoyl-β-ᴅ-glucose and Choline by a Cell-Free System from Developing Seeds of Red Radish (Raphanus sativus L. var. sativus)