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In the present investigation different finestructural and histochemical procedures were employed to demonstrate normal morphology and Ca 2+ -transport mechanism in bullfrog atrial myocardium. For normal morphology specimens were fixed in 1% OsO 4 , 2.5% glutaraldehyde or liquid propane at - 185 °C before they were prepared for conventional embedding and freeze-etching, respectively. Special interest was focussed on the caveolae system, which is composed of single, spherical membrane invaginations (diameter, 85 nm), randomly distributed at the entire cell periphery. The caveolae enlarge the cell surface by 24.5% and occupy 8.5% of the cellular volume. The caveolae membrane contains a few intramembraneous particles with a diameter of 8.4 nm comparable to the size of ATPases as found in other cells.

Histochemical Demonstration of an ATP-Dependent Ca 2+ -Pump in Bullfrog Myocardial Cells