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The binding of the dye cations acriflavine AF, tetramethylacriflavine TMAF and acridine orange AO (scheme of structures) to calf thymus DNA has been investigated by means of absorption spectroscopy, Table I. In order to avoid dye association we used very low dye concentrations and sufficiently high DNA concentrations. In this case we got linear Scatchard isotherms. The formal Scatchard binding constant K strongly depends on the salt concentration Cs (S = NaCl) of the solution and the temperature T (278 - 303 K), K (C S , T). The average value of binding sites per mononucleotide is n = 0.17. It is independent of the dye species and of C S  and T.

zeitschrift für naturforschung c

Bindungskonstanten, Bindungsenthalpien und Entropien für die nicht-kompetitive und die kompetitive Bindung von Acriflavin, Tetramethylacriflavin und Acridinorange an DNA /  Binding Constants, Binding Enthalpies and Entropies of the Non-Competitive and the Competitive Binding of Acriflavine, Tetramethylacriflavine and Acridine Orange to DNA