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The effects of the aminotriazinone herbicides metribuzin [4-amino-6-tert-butyl-3-(methylthio)-1,2,4,-triazin-5(4H)one] and metamitron [4-amino-3-methyl-6-phenyl-1,2,4-triazin-5(4H)-one] on the metabolism of enzymatically isolated leaf cells of Abutilon theophrasti Medic, were examined. Photosynthesis, protein, ribonucleic acid (RNA), and lipid synthesis were assayed by the in­ corporation of radioactive substrates such as NaH 14 CO 3 , [ 14 C]leucine, [ 14 C]uracil, and [ 14 C]acetate, respectively, into the isolated cells. Photosynthesis was the most sensitive process inhibited by both herbicides, but metribuzin was a more potent inhibitor than metamitron. Protein synthesis was not affected by any herbicide. RNA synthesis was inhibited significantly by both herbicides and may be involved in their ultimate herbicidal action. Lipid synthesis was inhibited significantly only by the high concentrations of metribuzin, and it was not affected by any concentration of metamitron at any incubation time. Inhibition of lipid synthesis may be involved in the action of metribuzin as a herbicide.

Use of Isolated Leaf Cells of A butilon theophrasti to Localize the Action of Two Aminotriazinone Herbicidal Derivatives