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The calcium-transport-ATPase of the sarcoplasmic reti­culum membranes is irreversibly inactivated by the combined action of Lasolocid and Triton X-100 at concentrations which separately do not interfere with the enzyme's activity. In the presence of Lasolocid the enzyme is most susceptable to inactivation when the Triton X-100 concentration just exceeds its critical micellar concentration, ~ 0.2 mg · ml -1 . Lasolocid becomes effective at a concentration of 10 μᴍ and produces rapid inactivation at 100 μᴍ . The enzyme is more rapidly inactivated in the active than in the inactive state.

Inactivation of the Sarcoplasmic Reticulum Calcium -Transport-ATPase by Lasolocid in Combination with Triton X-100