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1-anilino-naphthalene-8 -sulfonate (ANS) fluorescence measurements have revealed that red blood cell membrane of the Rh null  type undergoes a transition at about 16 °C. In contrast, viscosity measurements of the extracted membrane lipids showed the usually observed transition at about 18 °C.

Different Protein-Lipid Interaction in Human Red Blood Cell Membrane of Rh Positive and Rh Negative Blood Compared with Rh null