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ᴅʟ-[β- 14 C] 3,4-Dihydroxyphenylalanine (DOPA), [2- 14 C]pyruvate and [2- 14 C]acetate were ad­ministered to a callus culture of S. hassjoo and incorporation of the radioactivity into 3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (I) and 1-methyl-3-carboxy-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline (II) was examined. Incorporation of radioactivity from labelled DOPA into I and II, and from acetate into I were observed, while that from pyruvate into I and II and from acetate into II were hardly detected. The biosynthetic pathways of I and II were discussed.

zeitschrift für naturforschung c

Biosynthesis of 3-Carboxy-6,7-dihydroxy-1, 2,3,4-tetra- hydroisoquinoline and 1-Methyl-3-carboxy-6,7-dihydroxy-1, 2,3,4- tetrahydroisoquinoline in a Callus Culture of Stizolobium hassjoo