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The fluorescent staining of fixed A9 cells (mouse fibroblasts) with ethidium bromide E has been investigated qualitatively and quantitatively. E is bound to DNA, RNA and protein. After enzymatic RNA digestion and staining at pH = 4.0 the dyestuff is bound to DNA specifically. The formed E-DNA-complex is stoichiometric if DNA will be saturated by high dye concentra­tion, C F = 1.0 × 10 -2  ᴍ. The stoichiometric factor n = 0.21 was determined from Scatchard bind­ing isotherms and by spectrophotometric titration.

zeitschrift für naturforschung c

Über die quantitative fluorometrische Bestimmung von DNA mit Ethidiumbromid in der Zelle und die Konstanz der Quantenausbeute des Ethidium-DNA-Komplexes im biologischen Milieu / On the Quantitative Fluorometric Determination of Cellular DNA by Ethidium Bromide and the Constancy of the Quantum Yield of the Ethidium -DNA -Complex in the Biological Environment