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Lecithin vesicles containing different cations were prepared by sonication and characterized. When the concentration of the divalent cations in the buffer was increased from 1 mᴍ to about 100 mᴍ the cation-concentration inside of the vesicles did not increase proportional to the outside concentration, but showed a saturation behaviour. The efflux of various cations mediated by ionophore A 23187 was measured and relative transport-rates were determined. The following sequence was obtained:

The Specificity of Ionophore A 23187 in Cation Transport across Lipid Membranes Studies with Lecithin Vesicles