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2-Hydroxymuconic acid, formerly postulated as an in­termediate of the bacterial degradation of chloridazon (5-amino-4-chlor-2-phenyl-2H-pyridazin-3-on), could be isolated after incubation of 5-amino-4-chlor-2-(2,3-dihydroxy-phenyl)-2H-pyridazin-3-on (1) with purified metapyrocate-chase and enriched am idase from chloridazon-degrading bacteria by reversed phase high performance liquid chromatography.

zeitschrift für naturforschung c

Enzymatische Bildung und Isolierung von 2-Hydroxymuconsäure, ein Metabolit im bakteriellen Abbau des Herbizids Chloridazon / Enzymatic Formation and Isolation of 2-Hydroxymuconic Acid, a Metabolit in the Bacterial Degradation of the Herbicide Chloridazon