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The binuclear electron paramagnetic resonance signals of α-methemocyanin of Helix pomatia were investigated at liquid helium temperatures. These signals were completely eliminated by the addition of fluoride and enhanced in the presence of chloride, which did not alter the saturation behavior. The triplet nature of the signal at g = 4.59 was confirmed by the low power-saturation. The addition of nitrite at pH 5.0 and 0° C yielded slowly and reversibly a seven-line half-field signal. On lowering the temperature the intensity of the triplet signals increased to the lowest temperature reached 4.2 K, excluding a strong exchange coupling.

Binuclear Copper Electron Paramagnetic Resonance Signals of α-Methemocyanin of Helix pomatia