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The water plant Lemna perpusilla has been incubated with the E. coli plasmids pMB9 and pBR325, respectively. Uptake of plasmids has been shown by subsequent trans­ formation of E. coli cells to tetracycline resistance after treatment with Lemna DNA from plasmid-incubated plants. In 7 out of 15 assays we found stable transformants. From the transformation rate an amount of 10 -4  to 10 -6  μg plasmid DNA per 10 μg of plant DNA can be calculated.

Evidence for Uptake of Plamid DNA into Intact Plants (Lemna perpusilla) Proved by an E. coli Transformation Assay