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Tobacco cells grown photoheterotrophically with high ammonium and sulfate concentrations are able to cope with small amounts of methionine-sulfoximine. The observed methionine-sulfoximine tolerance of tobacco suspensions is due to an extracellular glutathione and an intra­ cellular glutamine reservoir, both reservoirs are considerably reduced by treatment with methionine-sulfoximine concentrations that do not affect the growth of the cells. In tobacco suspension cultures grown with nitrate as sole nitrogen source that do not contain high amounts of glutathione and glutamine, growth inhibition by methionine-sulfoximine can be prevented by addition of these substances to the growth medium. These data indicate that synthesis of glutathione and glutamine are both inhibited by mentionine-sulfoximine; furthermore they show evidence that - in contrary to animal cells -the whole glutathione molecule is taken up by tobacco cells. Synthesis of glutathione from the consisting amino acids is inhibited by methionine-sulfoximine in crude cell homogenates to a similar extent than observed in tobacco suspensions in vivo; therefore, the activity, and not the amount of enzymes of glutathione syn­ thesis seems to be reduced by treatment with methionine-sulfoximine. As tobacco suspensions are able to recover from methionine-sulfoximine treatment with respect to accumulation of glutathione in the medium as well as with respect to growth, detoxication of methionine-sulf­ oximine has to be assumed.

Effects of ʟ-Methionine-S-sulfoximine on Growth and Glutathione Synthesis in Tobacco Suspension Cultures