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A sensitive fluorometric test for the determination of monooxygenase activity in liver microsomes from rats is described. The assay is based on the O -demethylation of 6,7-dimethoxycoumarin (scoparone) to 7-hydroxy-6-methoxy-coumarin and 6-hydroxy-7-methoxycoumarin. Pretreatment of rats with phenobarbital or polycyclic hydrocarbons (benzo[a]pyrene, 3-methylcholanthrene) causes a significant increase in the amount of microsomal dealkylation activity.

zeitschrift für naturforschung c

Eine fluorometrische Bestimmungsmethode für mikrosomale Monooxygenase-Aktivität der Rattenleber mit Scoparon als Substrat / A Fluorometric Test for Microsomal Monooxygenase Activity in the Rat Liver with Scoparone as Substrate