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Cell lines derived from type II lung cells were used to study interplays of substances affecting incorporation of labeled precursors [1- 14 C] palmitate and [methyl- 3 H] choline into phosphatidyl choline. Ethanol stimulated markedly biosynthesis of dipalmitoyl phosphatidyl choline in cloned rabbit lung cells; the stimulating action of ethanol was reduced very much by cortisol and less by ritodrine. In the presence of 0.1 μᴍ isoproterenol, two prostaglandins, E 2  and F 2a , caused marked depressions in the incorporation of both precursors by cell line A 549 derived from human lung adenocarcinoma. One concluded that among the agents studied, ethanol and cortisol are potent antagonists, and so were also the prostaglandins and isoproterenol.

Cultured Lung Cells: Interplay Effects of Beta-Mimetics, Prostaglandins and Corticosteroids in the Biosynthesis of Dipalmitoyl Lecithin