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Infection of tobacco protoplasts with RNA of tobacco mosaic virus and the bromoviruses has been studied. The quality of the RNA was very important. Ribonuclease activity was controlled by incorporating 5 mᴍ ZnCl 2  in the inoculum. Up to 60% infection has been observed with bromovirus RNA but infection with TMV RNA was much less efficient. A procedure for inoculating in 40% polyethylene glycol was developed. High levels of infection were obtained with all the viruses and viral RNA examined.

Improved Methods for Infection of Plant Protoplasts with Viral Ribonucleic Acid