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A method is described for the estimation of the pigment content in chloroplasts by means of HPLC. The method is highly sensitive. Less than one picomole of individual pigments - equivalent to 1 milligramme fresh weight of spinach leaves - is needed for quantitative analysis. Analysis time is only 15 min, whereas in routine determination by thin layer chromatography a single sample needs 4 hours to be completed.

zeitschrift für naturforschung c

Die quantitative Bestimmung von Chloroplastenpigmenten im picomol-Bereich mit Hilfe einer isochratischen HPLC-Methode/ A Method for the Quantitative Estimation of Chloroplast Pigments in the Picomole-Range by Use of a Isocratic HPLC-System