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Further investigations on the structure of Friend murine leukemia virus (FLV) revealed that the transition from the “immature” (now termed “native”) to the structurally less organized “mature” (now termed “collapsed”) form occurs mainly as a result of the preparation for the electron microscope. A short pretreatment of virus with the detergent NP40 prior to negative staining with uranyl acetate is able to preserve the “native” structure of a high percentage of virus particles from standard preparations. Treatment with conventional fixatives was found to be in ­ effective.

zeitschrift für naturforschung c

Properties of Mouse Leukemia Viruses X V . Electron Microscopic Studies on the Organization of Friend Leukemia Virus and Other Mammalian C-Type Viruses