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The recombination of chloroplast coupling factor 1 (CF 1 ) and thylakoid membranes in reconsti­ tution experiments was studied through the fluorescence of paired labels covalently bound to NH 2- groups. It was found that maximum recombination is achieved at a ratio of 1.5 -3,μg CF 1  /μg chlorophyll. The addition of chloroplast lipids to the medium enhances the incorporation of CF 1  into the membranes.

A Fluorescence Method for Measuring the Retention of Coupling Factor (CF 1 ) in Reconstitution Experiments of Photophosphorylation