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An improved method is described for the separation of 22 compounds normally related to purine and 6-thiopurine metabolism in biological materials using high-pressure liquid cation-exchange chromatography on strongly acidic exchange resin. The column (0.18 × 100 cm) is eluted with 0.4 ᴍ ammonium formate, pH 4.6, at a linear flow velocity of 5.2 cm · min-1 at 50 °C. The elution volumes of sulphate anions, allopurinol, 6-thioxanthine, adenine, adenosine, and guanosine are demonstrated additionally to further 16 purine and 6-thiopurine compounds.

zeitschrift für naturforschung c

IV. An Improved Separation Method for Twenty two Compounds Related to Purine and 6-Thiopurine Metabolism Using High-Pressure Liquid Cation-Exchange Chromatography: The Quantitative Determination of Metabolites of 6-Mercaptopurine in Biological Materials