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Juvenile Hormone, Manduca sexta The juvenile hormones (JH) in extracts of M anduca sexta blood were converted to 10-(2,4-di-chlorobenzoyloxy)-11-methoxy derivatives and quantitatively determined by gas chromatography with electron capture detection. Early IVth instar larvae contained 0.62 ng JH-I, 1.1 ng JH-II and 0.07 ng JH -III per ml blood. Early Vth instar larvae, and adult of both sexes contained the hormones in concentrations from 0 to 0.24 ng/ml blood. No hormone was detected in late Vth instar (wandering) larvae. The detection limit in our experiments was ≦ 0.003 ng/ml blood.

The Juvenile Hormones in Blood of Larvae and Adults of Manduca sexta (Joh.)