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The size of poly(A)-RNA from polysomes and cell nuclei of the yeast Saccharomyces cerevisiae was investigated. Pulslabelled cells ([ 14 C]adenin) were cracked by the French press; polysomes and nuclei were separately isolated and the RNA was finally extracted with phenol. The separation of poly (A)-containing and poly (A)-lacking fractions was achieved by oligo (dT) cellulose. These fractions were characterized by sedimentation analysis. The main portion of polysomal poly (A) -RNA sedimented with a rate of 8 to 14S, whereas the poly (A)-RNA of nuclei exhibited a sedimentation rate of 12 to 17S. Thus nuclear poly (A)-RNA is about 20 -30% larger than polysomal poly (A) -RNA.

zeitschrift für naturforschung c

Größenvergleich der Poly(A)-RNA aus Polysomen und aus Kernen bei der Hefe Saccharomyces cerevisiae / Size Comparison of Poly (A )-RNA from Polysomes and from Nuclei of the Yeast Saccharomyces cerevisiae