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To measure pO 2  or pCO 2  in gases or fluids the fluorescence indicators pyrene butyric acid or β-methylumbelli-feron were used, which were separated from a measuring medium by a gas-permeable membrane. For p CO 2  the pH changes in NaHCO 3  solution were monitored by methyl-umbelliferon. Changes in its fluorescence signal, S, were measured at λ (emission) = 445 nm. Excitation was brought about by light of wavelength λ 1  = 318 nm (fluorescence signal S 1 ) , and λ 2  = 357 nm (fluorescence signal S 2 ). Drawing lg(S 2 /S 1 ) against lg(pCO 2 -1), one obtains a straight line between a p CO 2  of 10 and 70 Torr. The response time is about 3 to 4 s (90%). For pO 2  , pyrene butyric acid is used (λ (excitation) = 342 nm; λ(emission) = 395 nm). A linear increase in pO 2  between 0 and 300 Torr yields a linear increase in the reciprocal fluorescence signal. The response time is about 3 s (90%).

zeitschrift für naturforschung c

Die pCO 2 -/pO 2 -Optode: Eine neue p CO 2 - bzw. pO 2 -Meßsonde zur Messung des pCO 2  oder pO 2  von Gasen und Flüssigkeiten / The pCO 2 -/pO 2 -Optode: A New Probe for Measurement of pCO 2  or pO 2  in Fluids and Gases