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Different preparative procedures for isolation and purification of plastocyanin were successful to obtain the pure protein from Chlorella vulgaris, Scenedesm us acutus and Spinacia oleracea but not from B um illeriopsis filiform is. Lade of plastocyanin in this alga was further confirmed by the following in comparison with spinach chloroplast material:

Absence of Plastocyanin in the Alga Bumilleriopsis and its Replacement by Cytochrome 553