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A biospecific adsorbent for the separation of different nucleic acids was prepared by covalent attachment of a chromophore analog of actinomycin C 1  (D) to Sepharose 4 B. The immobilized chromophor-derivative was obtained by condensation of 2-nitro-3-hydroxy-4-methylbenzoic acid with N 6 -(6-aminohexyl)-Sepharose 4 B, reduction of the nitro group and oxidative coupling with 2-amino-3-hydroxy-4-methylbenzoyl-β-(N,N-diethylamino) -ethylamide. Since the chromophore of actinomycin has a high affinity to DNA. DNA/RNA-mixtures were separated by retardation of DNA on this resin

zeitschrift für naturforschung c

Trägergebundenes Analog des Actinomycins C 1  (D) und Affinitäts­ chromatographie von Nucleinsäuren / Resin Linked Analogue of Actinomyein C 1 (D) and Affinity Chromatography of Nucleic Acids

Trägergebundenes Analog des Actinomycins C 1 (D) und Affinitäts­ chromatographie von Nucleinsäuren / Resin Linked Analogue of Actinomyein C 1 (D) and Affinity Chromatography of Nucleic Acids

Trägergebundenes Analog des Actinomycins C 1 (D) und Affinitäts chromatographie von Nucleinsäuren / Resin Linked Analogue of Actinomyein C 1 (D) and Affinity Chromatography of Nucleic Acids