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Long wavelength UV irradiation (302/313 nm) of hybrid and bifilarly labeled BrU-DNA of B. subtilis results in a degradation of the molecular weight of BrU and of parental thymine con­ taining DNA strands. Hydrogen donors present during UV irradiation are able to prevent these “primary” and “secondary” strand breaks. The protection factors are especially large for secondary breaks in parental normal DNA. Through the action of protective agents the biological activity could not be restored. The generation of these primary and secondary strand breaks due to BrU incorporation and the action of hydrogen donors discussed.

zeitschrift für naturforschung c

The Influence of Hydrogen Donors on Breakage of Parental DNA Strands and on Biological Activity of Transforming BrU-DNA of B. subtilis after 302/313 nm Radiation