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climate chamber at 28 °C, 85% relative humidity and continuous illumination with white fluorescent light (Belalux, Osram G m bH *), 20 tubes/m2, distance from the object 110 cm and intensity ap­ proximately 3000 lux. After three weeks of culture the grown colonies were resuspended with liquid medium and the colonies per 0.1 ml of the resulting suspension were counted and the plating efficiency was calculated. One factor which influences the plating efficiency of protoplasts has been shown to be the intensity of the illumination during the first two days of culture following isolation. The effects of different illumina­ tion in the development of calli are compared in Table I. One fraction of the protoplast preparation was continuously maintained in 400 lux. Another fraction (the control) was continuously maintained in 3000 lux, and the third fraction was maintained in 400 lux during a period of 48 hours and was then transferred to 3000 lux. Cultures maintained three

zeitschrift für naturforschung c

Über Flavon- und Anthrachinon-Farbstoffe der Digitalis-Arten, V 1  . Die Struktur von Digiferrol und Digiferruginol / About Flavone and Anthraquinone Pigments of Digitalis. The Structure of Digiferrol and Digiferruginol