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Fragments of the thylakoid membrane, obtained by ultrasonication of stroma-freed chloroplasts and subsequent fractioning centrifugation, aggregate in buffer solutions of suitable ionic strength in the pH-range of 6.0-8.5. This aggregation leads to the formation of vesicles and membranes, which may reach a size of up to 0.5 mm. A suspension of the fragments, having an average diameter of 100 Å, is stable in water.

Membrane and Vesicle Formation from Fragments and Proteins of Thylakoids