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In order to realize real-time monitoring of Cu 2+ , a new fluorescent probe  HL , a Schiff base derivative of  N - n -butyl-4-[2]-1,8-naphthalimide, has been designed and synthesized. In methanol-HEPES [2-(4-(2-hydroxyethyl)-1-piperazinyl)-ethanesulfonic acid] solution (1:1,  v / v , pH = 7.4)  HL  showed excellent selectivity towards Cu 2+  over other common coexisting metal ions. The fluorescence intensity for  HL  showed a good linearity with the concentration of Cu 2+  ions in the range of 0.5–5.0 μ m . Based on combined fluorescence titration, Job’s plot analysis, and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry results, Cu 2+  forms a 1:2 complex with  L . The fluorescence intensity of  HL  exhibits significant quenching after binding with Cu 2+ , owing to the strong, intrinsic paramagnetic behavior of Cu 2+ . Ultimately, in order to test the performance of the synthesized probe,  HL  was preliminarily applied to the determination of Cu 2+  in the Yellow River and in tap water with satisfying results.

A selective fluorescence probe for copper(II) ion in aqueous solution based on a 1,8-naphthalimide Schiff base derivative