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Three new steroidal saponins were isolated from the roots of Smilax officinalis Kunth. Their structures were established as (3β,5β,22α,25S)-26-(β-D-glucopyranosyloxy)-22-hydroxyfurostan- 3-yl O-α-L-arabinopyranosyl-(1→6)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside, (3β,5α,22α,25S)-26-(β-D-glucopyranosyloxy)-22-hydroxyfurostan-3-yl O-α-L-arabinopyranosyl-( 1→6)-O-[β-D-glucopyranosyl-(1→4)]-β-D-glucopyranoside and (3β,5α,6β,22α,25S)-26-(β- D-glucopyranosyloxy)-6,22-dihydroxyfurostan-3-yl O-α-L-arabinopyranosyl-(1→6)-O-[β-D-glucopyranosyl-( 1→4)]-β-D-glucopyranoside. Their structure elucidations were performed using detailed analyses of  1 H and  13 C NMR spectra including 2D NMR spectroscopic techniques (DEPT, COSY, HSQC, HMBC and HMQC) and chemical conversions.

Three New Steroidal Saponins from Smilax officinalis