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The four component condensation (4 CC, Ugi reaction) of aqueous alginic acid with formaldehyde, difunctional amine and isocyanide components produces a gel. Enzymes, e.g. “acidic phosphatase” (AP) and L-(+)-lactate dehydrogenase (LDH), can be immobilized by inclusion in the above crosslinked alginic acid. The enzymes retain some of their activity (LDH: 8-10%; AP: 82-87%).

zeitschrift für naturforschung b

Vernetzung wäßriger Alginsäure mittels der Vierkomponenten-Kondensation unter Einschluß-Immobilisierung von Enzymen / Crosslinking of Aqueous Alginic Acid by Four Component Condensation with Inclusion Immobilization of Enzymes