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We want to report on syntheses and test reactions of some ferrocenic compounds which could be used as derivatizing reagents for peptides and proteins in order to detect them electrochemically after an HPLC analysis run. Among the tested compounds we found the 3-ferrocenylpropionic anhydride as the most suitable reagent for this purpose. It is a stable compound which can be prepared with high yields from 3-ferrocenylpropionic acid. Its reaction products with peptides and proteins are highly electroactive which is essential for the sensibility and selectivity of the method.

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Derivatisierungsreagenzien für die HPLC-ECD-Analyse von Peptiden und Proteinen auf Ferrocenbasis: Synthese und Umsetzung mit H – Phe – O t Bu als Eignungstest / Derivatizing Reagents to Analyse Peptides and Proteins by HPLC-ECD Based on Ferrocene: Synthesis and Reaction with H – Phe – O t Bu to Test their Suitability