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Pyrolysis mass spectrometry is shown to be a useful tool in the analysis of the protected intermediate deoxy oligonucleotides synthesized by the phosphotriester approach. Characteristic ions of the protected nucleic acid bases and terminal substituents appear in the mass fragmentation pattern which allows a rapid control of the attachment of the polynucleotide block in the chemical synthesis.

Pyrolysis Mass Spectrometry of Protected Phosphotriester Oligodeoxyribonucleotides