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The (Z,Z)-configuration of the bilivioline chromophoric system was established investigating an appropriate model compound by means of pK measurement,  1 H NMR spectroscopy Lanthanide ion induced shift technique, electron absorption spectroscopy and PPP calculations. Moreover a syn-syn conformation which is twisted at the a-methine single bond and planar within the pyrromethene unit (based on the concept of partial structures of bile pigments) of the molecule was deduced.

zeitschrift für naturforschung b

Beiträge zur Chemie der Pyrrolpigmente, XVII / On the Chemistry of Pyrrole Pigments, XVII: Struktur- und konformationsanalytische Untersuchungen am chromophoren System der Bilivioline [Biladiene-(a.b)] / Investigations of Structure and Conformation of the Chromophoric System of Biliviolines [Biladienes-(a, b)]