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The analysis of relationships between structure and retention time in gas liquid chromatography of pentoses and hexoses leads to the formulation of following rule: the per-O-trimethylsilyl derivatives of aldoses stereoisomers with conformational stability, identical ring form and the same number of substituted OH groups are longer retained on polar columns when the equatorial substituents are more numerous and are located nearer to the anomeric carbon.

The Effect of Stereoisomeric Structure on Retention Time in Gas Liquid Chromatography of Per-O-trimethylsilyl Derivatives of Pentoses and Hexoses