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We investigated the behaviour of RNA from the cotyledons of sterile mustard seedlings (Sinapis alba L.) under conditions were Pf r , i. e. the active phytochrome, will rapidly mediate enzyme induction, e. g. phenylalanine ammonia lyase induction. Using double labelling technique of RNA ( 14 C-uridine,  3 H-uridine) and separation of the RNA on MAK 1 -columns we could not find any change of the RNA fractions within 20 min after the onset of light, i. e. within 20 min after the formation of Pf r  in the seedling. The results indicate that the MAK-method of RNA separation is not precise enough to reveal small differences at the level of rapidly labelled RNA. Positively, we conclude that Pf r  mediates morphogenesis not by dramatic regulatory changes but by induction and repression of a relatively small number of enzymes  2 .

zeitschrift für naturforschung b

MAK-Chromatographie der RNS im Zusammenhang mit der phytochromgesteuerten Morphogenese / MAK-Chromatography of RNA in Connection with Phytochrome-Mediated Morphogenesis