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1. The stability of NNMG with respect to pH was investigated. A maximum, with a half-life of 40 hours, was found in phosphate-citric acid buffer at pH 5. The stability decreases quickly with increasing concentration of hydroxyl ions. At the optimal mutation rate (pH 6, 37°, and shaking) the half-live is only 14,4 hours.

zeitschrift für naturforschung b

Zum Wirkungsmechanismus von 1-Nitroso-3-nitro-1-methylguanidin (NNMG) bei der Mutationsauslösung / The Mode of Action of 1-Nitroso-3-nitro-1-methyl-guanidine (NNMG) in Mutagenesis: IV. Beständigkeit des NNMG, Beziehungen zwischen Mutationsrate und Aufnahme des Mutagens durch die Zelle und Förderung der Methylierung durch Sulfhydrylgruppen in Abhängigkeit vom pH-Wert