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We studied the molecular weight of cristalline GluDH from beef liver 1) in different dilutions, 2) after treatment with NADH and diethylstilbestrol. 3) after treatment with 6 M guanidine-HCl. When we diluted GluDH there was a spontaneous dissociation of the molecule into subunits. The molecular weight decreased from 2,000,000 (at 20 mg/ml) to 310.000 (at 0.01 mg/ml). After concentrating the solution again we got back the associated molecule. After treatment with NADH and diethylstilbestrol we obtained a dissociation of GluDH into subunits, too, but with a significant loss of enzymatic activity. This effect is reversibel — after dialysis we found the associated molecule again. When we treated GluDH with 6 M guanidine-HCl we found a complete dissociation of the molecule into subunits with a molecular weight of 52,000. This treatment caused an irreversibel loss of enzymatic activity.

zeitschrift für naturforschung b

Einfluß von Effektoren auf Molekulargewicht und Aktivität der Glutamat-Dehydrogenase (GluDH) aus Rinderleber in vitro.: I. Das Molekulargewicht der GluDH und ihrer Untereinheiten