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Actinomycin shows four effects on the synthesis and turnover of RNA in mammalian cells: in inhibits the synthesis of cellular RNA, it influences the base composition of newly synthesized RNA, it induces the degradation of a certain fraction of preformed RNA and it prevents the turnover of RNA synthesized in the presence of actinomycin. The first three activities are dose dependent. Synthesis of ribosomal RNA is inhibited by very low concentrations of actinomycin (0,005 μg/ml) whereby synthesis of messenger RNA continues.

Synthese und Umsatz von Ribonucleinsäuren in suspendierten Säugetierzellen: II. Einfluß von Actinomycin