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Ribosomal ribonucleic acid (rRNA) from rat liver has been investigated as to the stability of its secondary structure, using UV hyperchromism under heating at different ion conditions of the solvent. The T m  value is directly related to log [H®] within p H  5 -8 and at p H  7 to log µ of monovalent cations. In presence of monovalent cations (Li ⊕ , K ⊕ ) an inverse relation exists between ion radius and T m  value, while the T m  is directly related to the ion radius of bivalent cations (Mg 2⊕ , Ba 2⊕ ). Bivalent cations are more effective in stabilizing the secondary structure than monovalent ones of the same strength and radius.

zeitschrift für naturforschung b

Untersuchungen zur Struktur ribosomaler RNS aus Normal- und Tumorgeweben: III. Einfluß von p H , Ionenstärke, Ionenradius und Ionenvalenz auf die thermische Denaturierung