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An exo-β-N-acetylhexosaminidase is purified from bovine spleen homogenate. Several synthetic β-N-acetylglycosaminides and natural glycosides such as oligo-saccharides from chondroitin 4-sulfate-protein, glycopeptides from ovine submaxillaris mucin and mucopeptides from E. coli are found to be substrates for the enzyme. The mode of enzyme action, the influence of activators and competitive and noncompetitive inhibitors are investigated.

Reinigung und Eigenschaften einer β-N-Acetyl-o-hexosaminidase aus Rindermilz