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The fluorescence decay times (τ/τ 0 ) and quantum yields (η/η 0 ) of rhodamine 6G (donor) in their dependence on the concentration of malachite green (acceptor) in ethanol were measured for the donor to acceptor concentration ratios γᴅ/γᴀ 3.48 and 0.35. At fixed γᴀ, the values of τ/τ 0  and η/η 0  are markedly lower for the systems with higher γᴅ/γᴀ. The experimental results are compared with the theory of a multistep excitation energy transfer from donor to acceptor. Good agreement between theory and experiment was found for the critical distances R OD  = 60.7 Å and R OA  = 53.8 Å calculated from spectroscopic data. In the case of γᴅ/γᴀ = 0.35, the experimental results can also be properly described using the Förster-Galanin theory, whereas for the system with γᴅ/γᴀ - 3.48 the multistep energy transfer plays a significant role.

Effect of Acceptor Concentration upon Donor Fluorescence Decay and Quantum Yield