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Objectives The consumption of popular tobacco products has adverse effects on public health. Early diagnosis and treatment practices are essential based on the disease-symptom relationship in public health problems. There is a need to evaluate biochemical parameters to elucidate the pathophysiological mechanisms of these problems with experimental studies. We aimed to investigate the biochemical and physiological effects of cigarettes, hookahs, and electronic cigarettes (e-cigarettes) that people commonly use. Methods We have used Wistar albino rats, and the rats were exposed to cigarette smoke, e-cigarette smoke, and hookah smoke for 60 min/day for seven days. We detected malondialdehyde (MDA), nitric oxide (NOx), protein carbonyl oxidation (PCO), 8-hydroxy-2′-deoxyguanosine (8-OHdG), total antioxidant status (TAS), total oxidant status (TOS), reduced glutathione (GSH), and superoxide dismutase (SOD) levels in blood samples taken after the experiment. Results We observed that the redox balance was disturbed in all groups. E-cigarettes mainly triggered lipid peroxidation; only hookah activated the intracellular antioxidant system. Conclusions Cigarette, hookah, and e-cigarette smoking should be considered high-risk factors for individual and public health. The use of tobacco products adversely affects public health.

Redox-changing effects of popular tobacco products in rats