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Objective Despite the recent advances in diagnosis and treatment of hepatocellular carcinoma (HCC), it is still a major health problem. Therefore, understanding the molecular mechanism is very important. Our aim is to investigate the molecular basis of aflatoxin B1 (AFB1) induced HCC other than the hotspot  TP53  p.Arg249Ser (c.747G&gt;T) (R249S) mutation. Methods 525 genes previously reported to be involved in carcinogenesis with mutations in different cancer types were analyzed by next generation sequencing for 525 cancer-gene panel (Roche/NimbleGen) in one tumor sample (T29) and one cell line (MAHLAVU) carrying  TP53  R249S mutation. Additionally,  ARID2  and  BCORL1  genes were analyzed by Sanger sequencing for MAHLAVU and Primary Liver Carcinoma/Poliomyelitis Research Foundation/5 (PLC/PRF/5) cell lines. Results No other common gene mutations were found in the analyzed T29 and MAHLAVU samples and also no genetic variation possibly associated with AFB1 was detected in PLC/PRF/5 cell line and 68 COSMIC HCC samples. Likewise, no pathogenic mutation was detected in  ARID2  and  BCORL1  genes of MAHLAVU and PLC/PRF/5 cell lines. Conclusion No fingerprint mutations were detected in the analyzed genes. To the best of our knowledge, other hotspot mutations appear to be absent if not at a very low frequency in HCC carrying  TP53  R249S mutation.

turkish journal of biochemistry

Lack of hotspot mutations other than  TP53  R249S in aflatoxin B1 associated hepatocellular carcinoma: Aflatoksin B1 kaynaklı hepatosellüler karsinomlarda özgün TP53 R249S dışındaki mutasyonların eksikliği