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Background and objective Ethyl glucuronide (EtG) and ethyl sulfate (EtS), minor metabolites of ethanol, aid to forensic scientist and clinicians to determine recent ethanol uptake when ethanol elimination is occurred. Present study aimed to show (a) kinetics of EtG and EtS in blood and urine after 0.5 g/kg ethanol intake (b) peak concentrations and time of disappearance in Turkish people. Material and methods Seventeen (10 male, 7 female) healthy volunteers participated in the study. Blood and urine samples were obtained during 48 h after consumption and analyzed in LC-MS/MS. Results and conclusion Blood peak concentrations of EtG and EtS were 0.13–0.389 mg/L and 0.211–0.5 mg/L, urine peak concentrations were 6.89–30.42 mg/L and 10.5–58.17 mg/L, respectively. There was no EtG and EtS in all samples 24 h later in blood and 48 h later in urine. Correlation was found between EtG and EtS concentrations in blood. Most of obtained data are similar to literature, except EtS dominancy to EtG in urine and blood.

Time dependent change of ethanol consumption biomarkers, ethyl glucuronide and ethyl sulphate, after single dose ethanol intake