English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Objective Targeting the protein of interest to a particular tissue to achieve high-level expression is an important strategy to increase expression efficiency. The use of the plant seed oil body as a bioreactor can not only increase the amount of target protein, but also reduce the cost of downstream processing. Methods VEGF 165  was expressed in  Arabidopsis thaliana  seeds via oilbody fusion technology. The pKO-VEGF 165  vector was construted and transformed into  A. thaliana  seeds. T3 transgenic seeds was detected by SDS-PAGE and western blot methods. The cell activity was tested by MTT methods. Result The phaseolin promoter was used to drive seed-specific expression of the  VEGF  165    gene in transgenic  A. thaliana . The coding region of  VEGF  165    was fused to the  Arabidopsis  oleosin sequence to target the protein to the oil bodies in the seeds of transgenic plants. The T-DNA region of recombinant plasmid pKO-VEGF 165  was shifted to  A. thaliana  seeds via the floral-dip method. Protein was analyzed by electrophoresis and protein hybridization analyses. Finally, MTT assays showed that the oleosin-VEGF 165  fusion protein played a part in the proliferation of HUVEC cells in vitro. Conclusion Oleosin-VEGF 165  was successfully expressed and it had stimulated HUVEC cell proliferation activity.

Expression of a functional recombinant vascular endothelial growth factor 165 (VEGF 165 ) in Arabidopsis thaliana